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Mould proof packaging for mould proof experiment

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this standard specifies the anti mildew grade, technical requirements, environmental conditions of anti mildew packaging, test methods, inspection rules and marks, packaging, transportation, storage and other contents of packaging
this standard is applicable to the packaging of machinery, electricians, electronics, instruments and meters to prevent mold invasion during circulation
this standard is not applicable to the packaging of food, medicine and other products to prevent mold invasion during circulation. The provisions in the following documents become the provisions of this standard through the reference of this standard. For dated references, all subsequent amendments (excluding Corrigendum) or revisions are not applicable to this standard. However, parties to agreements based on this standard are encouraged to study whether the latest versions of these documents can be used. For undated references, the latest edition is applicable to this standard
GB /T 191 packaging, storage and transportation pictorial signs
GB /T 4122 packaging terms
GB /T 4797.3 natural environmental conditions of electrical and electronic products biological
GB /T 4798.2 environmental conditions for application of electrical and electronic products transportation
GB /T 5048 moisture proof packaging GB /T 4122 and the following terms and definitions are applicable to this standard
1 fungicide
chemical substances that can inhibit or kill the growth and development of mold. There are two categories of residual and volatile fungicides commonly used in industry
2 mold mold
is a kind of fungus. It is filamentous and can produce various forms of spores. It is mostly saprophytic and has many kinds. The common ones are Penicillium, Aspergillus and incomplete fungi. It can be used in industry to produce industrial raw materials, and some fungi can endanger human, animals, plants, industrial products and raw materials
3 spore
is the reproductive body of fungi, which is generally very small. It has various names due to its different characters, occurrence process and structure, such as asexual spore (conidia, sporangium, etc.), sexual spore (combined spore, sporangium, etc.). Spores that can resist adverse environmental conditions are called chlamydospores, dormant spores, etc. spores formed directly by vegetative cells through cell wall plus
thick and stored nutrients.
4 medium
can provide nutrients for mold growth, and generally contain nitrogen, carbohydrates and mineral salts (including trace elements) And water. According to the different raw materials used, it can be divided into natural medium and synthetic medium
5 simulation item
because the mold test is a destructive test, when valuable and complex packages cannot be reused after the test, or because the test sample is too large to provide large-scale test equipment, the test sample that can represent the material composition and manufacturing process of the product or package shall be used for the test. This kind of sample is called simulation. 1. When mould proof packaging is required for products, the grade requirements of mould proof packaging must be specified in the product technical documents
2 the anti mildew packaging grade of the package shall be determined according to the anti mildew ability of the product, the environmental conditions involved in transportation and storage, the package structure, the anti mildew performance of the selected packaging materials and the results of the mold test of the package
3 the level of mould proof packaging is divided into level 1 packaging, level 2 packaging, level 3 packaging and level 4 packaging. See Table 1 for the division of the four grades
Table 1 requirements for anti mildew packaging grade packaging grade: the inner and outer packaging materials and the surface of packaging parts of grade 1 packaging were tested for 28d according to this standard, and no mold growth was found
Level 1 packaging refers to the packaging that is often in zone B4 specified in GB /T 4797.3 or under corresponding environmental conditions (such as seaside, tunnel, etc.) within two years. In the process of transportation, it is often in zone 2B1 specified in GB /T 4798.2 or zone 2B2 and 2b3 with mold growth conditions, but the packaging has taken mold proof measures. After 28d mold test of level 2 packaging according to this standard, the inner packaging is well sealed, there is no mold growth on the surface of the product, and there is no mold growth on the surface of the inner packaging film. Mold grows in some areas of the outer package (composed of natural materials), and the growth area shall not exceed 10% of the total area of the whole package, but the service performance of the package shall not be affected by mold
Level 2 packaging is a package that is often in zone B2 and B3 specified in GB /T 4797.3 or under the environmental conditions corresponding to zone B2 and B3. After 28d mold test of level 3 packaging according to this standard, local mold growth occurred in the product and internal and external packaging. The moldy area shall not exceed 25% of the total area of the package
Level 3 packaging is applicable to the packaging in zone B1 specified in GB /T 4797.3 and zone 2B1 specified in GB /T 4798.2 or under corresponding environmental conditions. The package itself has not taken any anti mildew measures. After the 28d mold test of the packaging of grade 4 packaging products according to this standard, serious mold growth occurs locally or in the whole package, and the mold growth area accounts for more than 25% of the total area of the packaging. If the test is extended to 84d, the mechanical properties of the packaging materials will decline during the test period, resulting in mildew and affecting the appearance
Level 4 packaging is not suitable for long-term transportation and storage in hot and humid seasons between zones B2, B3 and B4 specified in GB /T 4797.3 or under corresponding environmental conditions. 1 quality requirements
1.1 carry out mould proof packaging design according to the nature, structure, storage, transportation, loading and unloading conditions of the packaged products and the production process of the packaged products, so that the product packaging can meet the requirements of mould proof packaging grade corresponding to the packaging requirements within two years after leaving the factory
1.1.1 when sealed packaging is adopted, the relative humidity in the packaging container or inner packaging bag shall be controlled within 60% within two years after leaving the factory, and the inspection of relative humidity shall be carried out in accordance with the requirements of moisture-proof packaging (GB /T 5048)
1.1.2 effective mould proof measures shall be taken for products with unsealed packaging to ensure that the products meet the mould proof requirements within two years after leaving the factory
1.2 the packaged products must be strictly inspected according to relevant regulations before packaging to confirm that the products are dry and clean, and there are no traces of mold growth and no pollution of organic substances that directly cause mold growth
1.3 when taking corresponding protective measures for product packaging according to the provisions of other professional packaging standards, it should be noted that the measures adopted should not have adverse effects on mildew prevention
2material requirements
2.1 the materials used shall be free from mildew and mildew spots before product packaging
2.2 materials used for mould proof packaging should be materials with strong mould resistance. Packaging materials in direct contact with products shall not use materials that will cause serious mildew
2.3 materials with poor mildew resistance shall be treated with moisture and mildew prevention according to corresponding standards and professional product technical requirements. The processed materials shall not produce corrosion and o
ther adverse effects on the packaged products
2.4 product packaging materials shall be dried according to relevant regulations before use. Avoid using materials with water absorption and moisture permeability exceeding the requirements
3 packaging environmental conditions
3.1 product packaging shall meet the requirements specified in GB /T 5048, especially the relative humidity in the packaging environmental conditions shall not exceed the specified range, keep the environment clean and avoid the medium conducive to the growth of mold into the packaging box
3.2 avoid polluting products and packages with organic pollutants such as hand sweat and grease during packaging. 1 test principle
this test method is to test in a test box (room) designed to simulate the environmental conditions of mold growth in nature and according to the physiological characteristics of mold growth, so as to evaluate the ability of packaging parts or packaging materials to resist mold invasion
this test method is applicable to the test of the extreme influence of mold on the packaging and packaging materials of products in the process of circulation, and is not applicable to the test of the extreme influence of mold on the packaging and packaging materials of food, medicine and other products in the process of circulation
2 test equipment
this test shall be carried out in a special mold test chamber (room), which shall meet the following technical requirements
2.1 the temperature of each point in the effective space of the test chamber (chamber) shall be between 28 ℃ ~ 30 ℃, and the temperature of the indicating point shall be controlled at 29 ℃ ± 1 ℃. 6.2.2 the relative humidity of the effective test space in the test chamber (room) shall be controlled to be greater than 96%, and the relative humidity of the indication point shall be controlled to be (97
± 1)%
2.3 the temperature fluctuation of each point in the effective space in the test chamber (chamber) shall not exceed 1 ℃ per hour
2.4 air change once every 7 days in the test chamber (chamber). During ventilation, the temperature in the box (chamber) shall not be lower than 25 ℃ and the relative humidity shall not be lower than 80%. The temperature at the indication point is allowed to fluctuate between 25 ℃ and 32 ℃, but it shall reach the values specified in articles 6.2.1 and 6.2.2 within 2h after ventilation
2.5 the wind speed in the test chamber is (0.5 ~ 2) m /s, but the wind speed shall not affect the normal growth of mold and meet the provisions of article 6.2.1
3 test procedure
this test must be carried out by professionals who master microbial operation technology
3.1 preparation of test samples
3.1.1 test samples can be tested for mildew resistance by selecting packages or representative packaging parts, simulators, inner packages and packaging materials according to the requirements of product standards and environmental conditions in the circulation process
3.1.2 the selected sample shall be consistent with the final use form of the product and can represent the packaging process of the package
3.1.3 in the whole process of test sample preparation, it must be ensured that the test sample is not artificially polluted
3.1.4 when the selected test samples are packaging materials, the quantity of each type shall not be less than 3 pieces
3.1.5 when the selected test samples are large samples such as packages or packaging parts, the number of samples shall be determined by both the entrusting unit and the testing unit through negotiation
3.2 preparation of test control sample
3.2.1 the control sample of sample is divided into the control test sample for detecting spore activity and the control test sample for determining the test conditions in the test chamber
3.2.2 the control test sample for testing spore vitality is a single spore suspension made of various strains used in the test, mixed into solid medium and cultured in a constant temperature incubator at 25 ℃. If various strains grow well within 7 days, the test is effective
3.2.3 the control sample for determining the test conditions in the test chamber is pure cloth strip or paper piece containing liquid culture medium. The samples prepared in 6.3.1 and 6.3.1 are randomly placed in the test box. If the mold of the control sample grows well within 7 days, the test is effective, otherwise the test must be repeated
3.3 preparation of test strains
3.3.1 the following eight kinds of molds should be used in the test of mould proof packaging. If the packaging materials or special packaging are tested, the corresponding strains with corrosion ability can be added, but they must be indicated in the report
Aspergillus niger v. Tieghem (A.C 3.392 8)
Aspergillus terreus Thom (A.C 3.393 5)
pullulans arnand (A.C 3.398 4)
Paecilomyces vannamei variot bamier (A.C 3.425 3)
Penicillium cordiformis Thom (A.C 3.387 2)
Penicillium ochraceus Biourge (A.C 3.430 2)
broom Thom (A.C 3.398 5)
Trichoderma virde pers Ex.Fx. (A.C 3.294 2)
3.3.2 the strains used in the test must be fresh strains cultured for 14 ~ 21 days
3.4 preparation of spore suspension
the preparation of spore suspension should be carried out according to the microbiological operation procedures. Using the strains specified in 6.3.3, put the re cultured spores into 200ml sterile water, and then add 1 drop of wetting agent (Tween 60 or Tween 80). The number of spores of each strain shall not be less than 0.5 × 10 /ml. Water droplets shall not be generated when spraying samples with spore suspension
3.5 test conditions
3.5.1 the mold box (chamber) for test shall be the test equipment that meets the technical index requirements of the mold box
3.5.2 during the test, the temperature of each point of the mold test chamber shall be between 28 ℃ and 30 ℃, and the relative humidity shall be the same

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